内含肽+intein是一种生物学术语，指的是一类特殊的蛋白质分子结构。内含肽是一种含有内跳肽的蛋白质序列，而intein则是指具有自身剪接和再连接功能的蛋白质片段。内含肽和intein的结合为生物技术和生物医药领域的研究提供了新的方向，尤其在蛋白质工程和基因组编辑领域有着广泛的应用。这些技术的发展不仅为基因调控和蛋白质设计提供了新的工具和方法，也为医学领域的疾病治疗和药物开发提供了新的可能性。以下是一些关于内含肽+intein的英语短句和例句，帮助读者更好地了解和应用这一领域的知识。
内含肽,intein
1)intein内含肽
1.Research and application advancement ofintein;内含肽的研究及应用进展
2.After the MAX-intein-CBD fusion protein was purified by chintin-affinity chromatography, the self-cleavage activity of theintein was induced by β-mercaptoethanol and the recombinan.用诱导剂IPTG诱导由目的多肽、内含肽和几丁质结合域(Chitin binding domain,CBD)组成的"三元"融合蛋白表达;用几丁质珠亲和层析纯化了裂解液中的融合蛋白,用β-巯基乙醇切割融合蛋白,获得目的蛋白。
3.In this study we explored theintein-mediated protein trans-splicing to deliver a Phe309→Ser mutant full-length fVⅢ (F309SfVⅢ) gene by using a dual-vector system.本文旨在利用内含肽(intein)的蛋白质反式剪接,研究双载体真核细胞转具有促分泌作用的F309S突变体全长fVⅢ(F309SfVⅢ)基因。
英文短句/例句
1.Study on self-splicing of split intein of the DNA polymerase DnaE in cyanobacteria蓝细菌分离DnaE内含肽的自我剪接研究
2.Intein-mediated F309SfVⅢ ligation with enhanced secretion of its heavy chain内含肽介导F309SfVⅢ的连接和重链分泌的增强
3.Large Protein Production Through Split Intein-Mediated Trans-Splicing通过断裂内含肽介导的反式剪接合成大的蛋白
4.Intein-mediated Trans-splicing of Chloride Ion Channel CFTR内含肽介导的氯离子通道蛋白CFTR的反式剪接
5.The Identification of Acidic Peptide in Animals Brain and the Analysis of Content in Big Mouse Brain;动物脑内酸性肽的鉴别和大鼠脑内含量的分析
6.Then to observe the change of GSH content through analyzing the rats"s anticoagulated blood.取抗凝血观察红细胞内谷胱甘肽含量的改变。
7.The Influence of Splenectomy to Glutathione of Red Blood Cells脾切除对红细胞内谷胱甘肽含量的影响
8.THE CORRELATION BETWEEN THE OPIOID PEPTIDE IN THE BRAIN AND THE MO NOCULAR LEARNING OF ONE-DAY-OLD CHICKS;雏鸡单眼学习与脑内阿片肽类含量的相关研究
9.EFFECT OF ACUTE HYPOXIA ON THE CONTENT OF B-EP IN THE BRAIN OF RATS高原急性适应过程中大鼠脑内β－内啡肽含量的实验研究
parison of Plasma ADH and β?EP Levels in the Internal Jugular Vein and Median Vein of the Elbow颈内与肘静脉血中抗利尿激素和β-内啡肽含量的比较
11.The Changes of Endogenous Opioid Peptides in Chronic Alcoholism Rats慢性乙醇中毒大鼠脑内内源性阿片肽系统含量的改变
12.TNA increased Glutathione S-Transferase (GST) activity and Glutathione (GSH) content obviously in soybean.作保灵能明显增加大豆体内谷胱甘肽含量和谷胱甘肽转移酶的活性。
13.EFFECT OF HYPERBARIC OXYGEN ON CONCENTRATION OF ENDOGENOUS OPIATE PEPTIDE IN SPINAL CORD TISSUES OF SPINAL CORD INJURY高压氧对脊髓损伤大鼠脊髓组织内源性阿片肽含量的影响
14.AN EXPERIMENTAL STUDY OF β-EP CONTENT IN PLASMA ON ACUTE CEREBRAL ISCHEMIA AND REPERFUSION脑缺血和重灌流不同时程血浆β-内啡肽含量的实验观察
15.Changes of ET and CGRP content in the lungs of the rats drowned in seawater海水淹溺大鼠肺脏内皮素及降钙素基因相关肽含量的变化
16.The Effect of Exercise Training on Central and Peripheral Beta-Endor-phin Concentration in Rats After Cool Stress运动训练对应激大鼠中枢和外周β-内啡肽含量的影响
17.High Density Cell Culture and Improvement of Glutathione Biosynthesis by Candida Utilis WSH 02-08;Candida utilis高密度培养及提高其胞内谷胱甘肽含量的研究
18.Effect of Electroacupuncture with Different Frequencies on β-Endorphin Contents in Cerebrums of Mice with Sarcoma;不同频率电针对荷瘤小鼠脑β-内啡肽含量的影响
相关短句/例句
DnaE inteinDnaE内含肽
3)intein蛋白内含肽
1.In order to obtain the recombinant Tp-5 cyclic analogue [cyclo-(Cys-Arg-Lys-Asp-Val-Tyr-),cTP]efficiently byintein-mediated single column purification, a gene encoding 6-amino acids peptide was designed, synthesized and cloned into Escherichia coli expression vector pTWIN1.为了实现蛋白内含肽(Intein)介导的重组环状胸腺五肽结构类似物[cyclo-(Cys-Arg-Lys-Asp-Val-Tyr-),cTP]的高效制备,设计并合成编码6个氨基酸的cTP基因,克隆到表达载体pTWIN1,重组表达质粒pTW-cTp转化E。
4)peptides containing lactone ring含内酯环肽
5)internal signal peptide内含信号肽
6)intein蛋白质内含肽
1.In this review, the discovery, characteristics, identification, splicing mechanism ofinteins and its biological significance were summarized.蛋白质内含肽是存在于前体蛋白质中的一段多肽链,靠自我剪切的方式从前体蛋白中释放出来。
2.Protein splicing, which is anintein mediated posttranslational processing, involves a series of intramolecular cleavage-ligation reactions.蛋白质剪接是蛋白质内含肽介导的,一种在蛋白质水平上翻译后的加工过程,它由一系列分子内的剪切-连接反应组成。
3.The progress in the mechanism of selfsplicing, structural characteristic, homing endonuclease and the application ofintein were summarized.蛋白质内含肽是能够自我剪接的一段多肽链。
延伸阅读
含内酯环肽分子式：CAS号：性质：肽链中的游离羟基与羧基以内酯键相连组成的抗生素统称为含内酯键的肽类抗生素。分为四个亚类：(1)放线菌素(actinomycin)类，由发色团2-氨基-4,6-二甲基吩噁嗪-3酮-1,9-二羧酸的两羧基，各引出一个含内酯环的五肽，约有百余种，由链霉菌产生，也有小单孢菌产生的。具有抗肿瘤作用，放线菌素D用于治疗何杰金氏病、神经母细胞瘤与绒毛膜上皮癌；(2)喹噁啉类，喹噁啉-2-羧酸的羧基与D-丝氨酸的氨基结合，丝氨酸的羟基与末端氨基酸的羧基形成内酯环，如左霉素(levomycin，actinoleukin，echinomycin)、醌霉素(quinomycins)、三骨霉素(triostins)等，有较强的抗革兰氏阳性菌和抗肿瘤活性，毒性大；(3)蜜柑霉素(mikamycin)-春霉素(vernamycin)类，3-羟基吡啶-2-羧酸与苏氨酸的氨基以酰胺键相连，苏氨酸的羟基与末端氨基酸的羧基形成内酯环，约有十余种，具有抗革兰氏阳性菌作用；(4)其他，如远霉素(telomycin)、蛎灰霉素(ostreogrycin)等，具有抗革兰氏阳性菌作用。
